Urushinge rwa chromatografmuri rusange koresha 1ul na 10ul. Nubwo inshinge zo gutera inshinge ari nto, ni ngombwa. Urushinge rwo gutera inshinge ni umuyoboro uhuza icyitegererezo nigikoresho cyo gusesengura. Hamwe nurushinge rwo gutera inshinge, icyitegererezo kirashobora kwinjiza inkingi ya chromatografi hanyuma kikanyura muri detector kugirango ikore isesengura rihoraho. Kubwibyo, kubungabunga no gusukura urushinge rwinshinge nibyo byibandwaho nabasesengura buri munsi. Bitabaye ibyo, ntabwo bizagira ingaruka kumikorere gusa, ahubwo bizanangiza ibikoresho. Igishushanyo gikurikira cyerekana ibice bigize urushinge.
Gutondekanya inshinge
Ukurikije isura y'urushinge, irashobora kugabanywamo inshinge zo gutera inshinge, inshinge zo gutera inshinge, hamwe ninshinge zo mumutwe. Inshinge zifatika zikoreshwa mugutera inshinge, zishobora kugabanya kwangirika kwa septum no kwihanganira inshinge nyinshi. Zikoreshwa cyane cyane mu gutera inshinge; inshinge za bevel zirashobora gukoreshwa kuri inshinge septa, byoroshye gukora. Muri byo, inshinge 26s-22 zirakwiriye cyane gukoreshwa kuri inshinge septa muri chromatografiya; inshinge zo gutera inshinge zikoreshwa cyane cyane kumashanyarazi no gutondekanya imiyoboro ya chromatografi ikora neza.
Ukurikije uburyo bwo gutera inshinge, irashobora kugabanywa inshinge zikora inshinge ninshinge zo gutera inshinge.
Ukurikije isesengura ritandukanye risabwa inshinge zatewe inshinge muri gazi ya chromatografi na chromatograf yamazi, irashobora kugabanywamo urushinge rwo gutera gaz hamwe ninshinge yo gutera inshinge. Urushinge rwa chromatografiya ya gaz rusanzwe rusaba inshinge nke, kandi ingano yo gutera inshinge ni 0.2-1ul, bityo urushinge rujyanye nubusanzwe ni 10-25ul. Urushinge rwatoranijwe ni urushinge rwo mu bwoko bwa cone, rworoshye gukora inshinge; ugereranije, ingano yo guterwa ya chromatografiya ni nini muri rusange, kandi ingano yo gutera inshinge ni 0.5-20ul, bityo ingano y'urushinge nayo nini nini, muri rusange 25-100UL, kandi inshinge y'urushinge iringaniye kugirango wirinde gushushanya stator.
Mu isesengura rya chromatografiya, urushinge rukoreshwa cyane ni urushinge rwo gutera inshinge, rukwiranye cyane cyane na chromatograf ya gaz hamwe nisesengura ryamazi ya chromatograf. Ubushobozi bwacyo bwose ni ± 5%. Imikorere yumuyaga irwanya 0.2Mpa. Igabanijwemo ubwoko bubiri: inshinge zo kubika amazi hamwe nububiko bwamazi. Urutonde rwibisobanuro bya micro-inshinge zidafite amazi ni 0.5μL-5μL, naho urwego rwerekana micro-inshinge ni 10μL-100μL. Urushinge rwa micro-inshinge nigikoresho cyingirakamaro.
Gukoresha inshinge
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. Iyo ukuyemo icyitegererezo, ibituba mumashanyarazi birashobora guhinduka hamwe nimpinduka ihagaritse yigituba.
(4) Mugihe ukoresheje inshinge, banza wuzuze inshinge amazi, hanyuma ukure amazi mumajwi asabwa.
Kubungabunga urushinge
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. Nyuma yiminota mike yo koza ubushyuhe, igikoresho cyo koza inshinge kirashobora kongera gukoreshwa.
Isuku y'urushinge
1. Urukuta rwimbere rwurushinge rwinshinge rushobora gusukurwa hamwe na organic organic. Mugihe cyo gukora isuku, nyamuneka reba niba inshinge yo gutera inshinge ishobora kugenda neza;
2. Niba inshinge yo gutera inshinge idasunika neza, inkoni yo gusunika irashobora gukurwaho. Birasabwa guhanagura neza hamwe nigitambara cyoroshye cyinjijwe mumashanyarazi.
3. Koresha inshuro nyinshi ibishishwa kama kugirango wifuze. Niba kurwanya inshinge zo gutera inshinge byiyongera byihuse nyuma yibyifuzo byinshi, bivuze ko hakiri umwanda muto. Muri iki gihe, inzira yisuku igomba gusubirwamo.
4. Niba urushinge rwo gutera inshinge rushobora kugenda neza kandi ruhamye, reba niba urushinge rwahagaritswe. Kwoza inshuro nyinshi urushinge hamwe na solge organic hanyuma urebe imiterere yicyitegererezo gisunikwa hanze y'urushinge.
5. Niba urushinge rwo gutera inshinge ari ibisanzwe, icyitegererezo kizasohoka kumurongo ugororotse. Niba urushinge rufunze, icyitegererezo kizaterwa mu gihu cyiza kiva mu cyerekezo kimwe. Nubwo ibishishwa rimwe na rimwe bisohoka mumurongo ugororotse, witondere kugenzura ko imigezi iruta iyisanzwe (gereranya gusa urujya n'urushinge rushya, rudafunze).
6. Guhagarika inshinge bizasenya imyororokere yisesengura. Kubera iyo mpamvu, gufata urushinge birakenewe. Koresha ikintu kimeze nkumugozi kugirango ukureho inshinge. Urushinge rushobora gukoreshwa gusa mugihe icyitegererezo gisohotse mubisanzwe. Gukoresha umuyoboro wifuza amazi cyangwa isuku ya siringi birashobora kandi gukuraho neza umwanda uri murushinge.
Kwirinda mugihe ukoresheje inshinge
Ntugafate urushinge rwa syringe hamwe nicyitegererezo hamwe nintoki zawe, kandi ntugire ibibyimba (mugihe wifuza, kuma buhoro, byihuse, hanyuma wifuze buhoro, subiramo inshuro nyinshi, 10 Ubunini bwurushinge rwicyuma rwa μl syringe ni 0,6 . syringe ifite intoki yumva iringaniye) Umuvuduko wo gutera inshinge ugomba kwihuta (ariko ntiwihuta cyane), ukomeze umuvuduko umwe kuri buri inshinge, hanyuma utangire gutera inshinge mugihe inshinge y'urushinge igeze hagati yicyumba cyuka.
Nigute ushobora kwirinda urushinge rwo gutera? Abashya benshi bakora isesengura rya chromatografi bakunze kunama inshinge ninkoni ya syringe. Impamvu ni:
1. Icyambu cyo gutera inshinge cyegeranye cyane. Niba isunitswe cyane ku bushyuhe bwicyumba, kashe ya silicone izaguka kandi ikomere igihe ubushyuhe bwicyumba cyuka kizamutse. Muri iki gihe, biragoye gushyiramo syringe.
2. Urushinge rwometse mugice cyicyuma cyicyuma cyo gutera inshinge mugihe umwanya utabonetse neza.
3. Inkoni ya syringe irunamye kuko imbaraga nyinshi zikoreshwa mugihe cyo gutera inshinge. Chromatografi itangaje, yatumijwe mu mahanga izana inshinge, kandi gutera inshinge ntibizunama inkoni ya syringe.
4. Kubera ko urukuta rw'imbere rwa syringe rwanduye, inkoni y'urushinge irasunikwa kandi ikunama mugihe cyo gutera inshinge. Nyuma yo gukoresha syringe mugihe runaka, uzasangamo ikintu gito cyirabura hafi yigitereko cyurushinge, kandi bizagorana kunyunyuza icyitegererezo no gutera inshinge. Uburyo bwo kweza: Kuramo inkoni y'urushinge, shyiramo amazi make, shyiramo inkoni y'urushinge mumwanya wanduye hanyuma usunike kandi ukurura inshuro nyinshi. Niba idakora rimwe, ongera utere amazi kugeza umwanda ukuweho. Muri iki gihe, uzabona ko amazi yo muri syringe aba mabi. Kuramo inkoni y'urushinge hanyuma uhanagure ukoresheje impapuro zungurura, hanyuma ukarabe n'inzoga inshuro nyinshi. Iyo icyitegererezo kigomba gusesengurwa nicyitegererezo gikomeye gishonga mumashanyarazi, oza syringe hamwe na solde mugihe nyuma yo guterwa.
5. Witondere gushikama mugihe utera inshinge. Niba ushishikajwe no kwihuta, syringe izaba yunamye. Igihe cyose uzaba umuhanga mu gutera inshinge, bizihuta.
Igihe cyo kohereza: Jun-19-2024