Nyamuneka hitamo ukurikije uko laboratoire yawe imeze.
Ni ngombwa gushakisha uburyo bwiza bwo guhanagura ibyombo
Kugeza ubu, hari umubare munini wibicuruzwa byubuhinzi byintangarugero (ibindi bicuruzwa bivura imiti, acide organic, nibindi) bigomba gupimwa na chromatografiya yamazi na gazi ya chromatografiya buri mwaka.Bitewe numubare munini wintangarugero, hari umubare munini wibikoresho byicyitegererezo bigomba gusukurwa mugihe cyo gutahura, bidatakaza umwanya gusa kandi bikagabanya imikorere yakazi, ariko kandi rimwe na rimwe bitera gutandukana mubisubizo byubushakashatsi kubera isuku ya inzabya zisukuye.
Icyitegererezo cya chromatografique gikozwe mubirahuri, gake gikozwe muri plastiki.Ikariso yintangarugero ikoreshwa ihenze, isesagura, kandi yanduza ibidukikije.Laboratoire nyinshi zisukura inzabya ntangarugero zikongera zikoreshwa.
Kugeza ubu, uburyo bukoreshwa muri laboratoire yo koza ibikoresho byongeweho cyane cyane byongeweho ibikoresho, ibikoresho byogeza, ibishishwa kama no gukaraba aside, hanyuma bigakaraba neza sisitemu ntoya.
Ubu buryo busanzwe bwa scrubbing bufite ibibi byinshi:
Gukoresha ibikoresho byogajuru bitwara amazi cyane, igihe cyo gukaraba ni kirekire, kandi hariho inguni bigoye gusukura.Niba ari plastike yintangarugero, biroroshye kugira ibimenyetso byohanagura imbere murukuta rwa vial, bifata abakozi benshi.Kubirahuri byanduye cyane ibisigazwa bya lipide na proteyine, umuti wa alkaline lysis ukoreshwa mugusukura, kandi ibisubizo byiza biragerwaho.
Iyo usesenguye ingero za LC / MS / MS, gusukura inzabya zatewe inshinge ni ngombwa cyane.Ukurikije uburyo bwo koza ibikoresho byibirahure, uburyo bwo gukora isuku bwatoranijwe ukurikije urugero rwumwanda.Nta buryo buhamye.Incamake yuburyo:
Icya mbere:
1. Suka igisubizo cyikizamini mumashanyarazi yumye
2. Shira ibisubizo byose byikizamini muri 95% alcool, kwoza kabiri ukoresheje ultrasonic hanyuma uyisuke, kuko inzoga zinjira byoroshye mumashanyarazi ya 1.5mL kandi birashobora kuba bibi hamwe numuti mwinshi wogukora kugirango ugere kubikorwa byogusukura.
3. Suka mumazi meza, hanyuma ukarabe ultrasonique kabiri.
4. Suka amavuta yo kwisiga mumashanyarazi yumye hanyuma uteke kuri dogere selisiyusi 110 mumasaha 1 kugeza 2.Ntuzigere uteka ku bushyuhe bwinshi.
5. Hisha kandi uzigame.
Icya kabiri:
1. Kwoza amazi ya robine inshuro nyinshi
2. Shyira muri beaker yuzuyemo amazi meza (Millipore imashini yamazi meza) na sonic muminota 15
3. Hindura amazi na ultrasound muminota 15
4. Shira muri beaker yuzuye Ethanol yuzuye (Itsinda rya Sinopharm, Analytical Pure)
5. Hanyuma, fata hanyuma ureke umwuka wume.
Ihitamo rya gatatu:
1. Banza ushire muri methanol (chromatografique yera), hanyuma usukure ultrasonique muminota 20, hanyuma usuke methanol yumye.
2. Uzuza amazi yicyitegererezo amazi, hanyuma usukure ultrasonique muminota 20, suka amazi.
3. Kama ibyombo by'icyitegererezo nyuma.
Icya kane:
Uburyo bwo koza ibyombo byintangarugero ni kimwe no gutegura icyiciro cyamazi, nibindi. Banza, koresha inzoga zubuvuzi kugirango ushire amasaha arenga 4, hanyuma ultrasound mugice cyisaha, hanyuma usukemo inzoga zubuvuzi, hanyuma ukoreshe amazi kuri kimwe cya kabiri cya ultrasound.Amasaha, kwoza amazi hanyuma uyumishe.
Ihitamo rya gatanu:
Banza, shyira mumashanyarazi akomeye ya okiside (potassium dichromate) mumasaha 24, hanyuma ukoreshe amazi ya deionised muri ultrasonic Karaba inshuro eshatu mubihe, hanyuma ukarabe na methanol rimwe, hanyuma uyumishe kugirango uyikoreshe.
Caps septas igomba gusimburwa, cyane cyane iyo isesengura ibisigazwa byica udukoko, bitabaye ibyo bizagira ingaruka kumubare.
Ariko niba ibintu bibyemereye, gerageza gukoresha ibintu bikoreshwa, nka PTFE yinjizwamo cyangwa winjizamo plastike yo murugo (hafi 0.1 yuan / igice), kandi vial sample ni nziza.Gukoresha inshuro nyinshi kandi ntukeneye kozwa.
Ihitamo rya gatandatu:
(1) bigoye inzira yisuku nibisubizo bifatika:
No1.Nyuma yikibindi cyicyitegererezo kimaze gukoreshwa, kwoza ibyombo byintangarugero ukoresheje amazi atemba, hanyuma uhanagure icyitegererezo gisigaye (urashobora kunyeganyeza intoki icyarimwe);
No2, hanyuma shyira vial sample muri potasiyumu dichromate yoza amazi menshi, kandi iyo yegeranije Iyo ugeze kumubare runaka cyangwa mugihe umeze neza, iyikure mumatungo yo kwisiga hanyuma uyashyire mumashanyarazi ya plastike mugikoni Koresha.Kwoza neza n'amazi ya robine.Urashobora gushungura inshuro nyinshi no kunyeganyega hagati;
No3.Koresha amazi ya robine kugirango usukure ultrasonique inshuro 3 nyuma yo koza.Hirya no hino, nibyiza kunyeganyeza amazi mumashanyarazi ntangarugero nyuma yo gukora isuku ya ultrasonic;
No4, hanyuma ukoreshe amazi atatu yatoboye (cyangwa amazi asukuye, amazi ya deionised) hamwe na 1.3 yoza ultrasonic inshuro eshatu;
No5, hanyuma ukoreshe chromatografique yera methanol ultrasonic yoza inshuro 2-3, nibyiza kuri
kunyeganyeza methanol mu bikoresho by'icyitegererezo nyuma yo gukora isuku;
No6.Shira inkono y'icyitegererezo mu ziko hanyuma uyumishe kuri dogere 80, kandi irashobora gukoreshwa.
(2) ibikoresho by'icyitegererezo byaguzwe kugirango ushireho amabara atandukanye:
niba wabonye ko hari ikimenyetso gito cyamabara kurugero rwicyitegererezo, kitari cyiza, ariko gifite imikoreshereze yacyo.Mugihe ugura, nibyiza kugura ibibindi byinshi byamabara atandukanye.
Kurugero: laboratoire yawe ifungura imishinga A na B icyarimwe.Igihe cyambere Umushinga ukoresha ibyitegererezo byera byera, naho umushinga B ukoresha icyitegererezo cyubururu.Ikizamini kimaze kurangira, gisukurwa ukurikije uburyo bwavuzwe haruguru, nubushakashatsi bwa kabiri Muri icyo gihe, koresha vial sample yubururu bwumushinga, umushinga wicyitegererezo cyera kumushinga B, nibindi, bishobora kwirinda neza ibibazo byatewe umwanda ku kazi kawe.
Andika kurangiza
1. Abashakashatsi benshi b'ibikoresho batanze igitekerezo: Koresha itanura rya muffle kuri dogere 400 kugirango uteke igice cy'isaha, ibintu kama byashize;
2. Shira ibikarito by'icyitegererezo mu itanura rya muffle kugirango wumuke kuri dogere selisiyusi 300.Injeniyeri wa Agilent ukomoka i Beijing yavuze ko ubwo yageraga mu itanura rya muffle, ikizamini ntikizaba urusaku nyuma yo guteka mu itanura rya muffle kuri dogere 300 mu masaha 6.
nanone ……… ..
Amashanyarazi mato mato, flasike imeze nk'isaro kugirango ihindurwe, hamwe nibindi bikoresho byibirahure kugirango bisesengurwe cyangwa byitondewe birashobora gusukurwa ukoresheje ubu buryo.
Igihe cyo kohereza: Gashyantare-25-2022