BitandukanyeHPLC IcyitegererezoIkizamini
Ibintu nibyiza niba wishyuye byinshi?
Ni irihe tandukaniro rya vial HPLC n'ibirango bitandukanye
Nka societe yumwuga ya sprometrike yumwuga, guhitamo ibikoreshwa mubigeragezo byahoze bishingiye kumibare.Mubigeragezo byubusa, hazajya habaho impinga yumuzimu idasobanutse, uhereye kumuti?Kuva kuri vial?Cyangwa ibicuruzwa bya plastiki biva mubikorwa byo kubikuramo?Twese tuzagenzura umwe umwe hamwe numwuka wo kumena casserole no gusaba imperuka.Kugirango tumenye neza ibizamini, twakoze urukurikirane rwo gusuzuma kuriicyitegererezo, gusa kugirango ubone imwe yizewe.Reba ibisubizo byacu byo gusuzuma, birashoboka ko nawe, nkurungano, nawe wahumekewe?
Ubwoko 11 bwamacupa yinshinge nubwoko 1 bwa intubation tube yitabira iri suzuma.Reka turebe uko amacupa yatewe inshinge asa ~~~
incamake
1. Ibikoresho by'icupa ryatewe inshinge birasa, isura irasa, kandi guhuza ibyiciro nibyiza.
2. Ibikoresho by'icupa ry'icyitegererezo / umuyoboro w'imbere ni plastiki n'ikirahure, kandi igiciro cy'ikirahure gihenze.
3. Igiciro cyibice bya plastiki yimbere yimbere icupa ryinshinge ni hafi 1 Yuan, kandi itandukaniro ryibiciro ntabwo ari rinini.
4. J ya plastike yimbere yubwoko D ikeneye gukoreshwa hamwe nuducupa twinshi -gutanga inshinge, zihenze kuruta icupa ryimbere rya plastike ryinjizwamo icupa.
5. Igiciro cyibice byikirahure cyimbere cyinjizwamo icupa ryatewe inshinge iri hagati yamafaranga 3 na 7, kandi ikinyuranyo cyibiciro ni kinini, kandi igiciro cyubwoko B cyumutekano nicyo kinini.
6. Igiciro cyibikoresho byo guteramo ibirahuri (hasi hasi, nta muyoboro wimbere) ni 0,6-2 yu, kandi igiciro cya A nicyo kinini.
Reba ibisubizo byikizamini gikurikira ~~ Imiterere yubushakashatsi
Ibikoresho byubushakashatsi: LC / MS (1290-6470) Icyiciro cya mobile: A: amazi meza B: acetonitrile Igipimo cy umuvuduko: 0.3ml / min
Uburyo: gusikana byuzuye muburyo bwiza nuburyo bubi Intambwe zigeragezwa:
Gupima neza ingano runaka y'amazi meza kandi
acetonitrile (urugero rw'icyitegererezo cy'icupa ryinjizwamo hamwe n'umuyoboro winjizamo hamwe n'umuyoboro winjizamo ni 100 μL, kandi urugero rw'icyitegererezo cy'icupa ryatewe nta muyoboro winjizamo ni 500 μL.) muri buri bwoko bw'icupa ryatewe, vortex kuri 1min, koresha ikirango kimwe nicyitegererezo (Agilent 5190-9024), hanyuma utere icyitegererezo kumashini.Kora scan yuzuye muburyo bwiza kandi bubi ion muburyo bukurikira.
Gutera inshinge + acetonitrile nziza ion uburyo
Icyitonderwa: Gitoya umubare wimisozi nuburebure bwimpinga, nibyiza.Amakuru yavuzwe haruguru arakoreshwa gusa, kandi ashinzwe gusa icyitegererezo.
Icupa ryatewe inshinge + amazi meza ion uburyo
Icyitonderwa: Gitoya umubare wimisozi nuburebure bwimpinga, nibyiza.Amakuru yavuzwe haruguru arakoreshwa gusa, kandi ashinzwe gusa icyitegererezo.
Guhitamo vial + acetonitrile mbi ion uburyo
Icyitonderwa: Gitoya umubare wimisozi nuburebure bwimpinga, nibyiza.Uwiteka
hejuru yamakuru ni ayerekanwe gusa, kandi ashinzwe gusa icyitegererezo.
Icupa ryatewe inshinge + amazi mabi ion uburyo
Icyitonderwa: Gitoya umubare wimisozi nuburebure bwimpinga, nibyiza.Uwiteka
hejuru yamakuru ni ayerekanwe gusa, kandi ashinzwe gusa icyitegererezo.
Imiterere y'urupapuro:
incamake
imikorere yimiti ya viza ya Spectrum J hamwe ninshinge ya A inshinge nibyiza, ariko ibyanyuma bifite gusubiramo nabi.Moderi ya B nayo yitwaye neza cyane, ariko igiciro cyari kuruhande rwo hejuru.Birasa nkaho ibintu bihenze byumvikana gato, ariko burigihe numva ko premium premium ari bike cyane.Ndabaza niba ubyumva kimwe ~~
Vuga muri make
ibirango bitandukanye by'amacupa yo gutera.Ibi bimenyetso bizatera ingaruka zitazwi mugushakisha intego zacu, ariko biragoye kubona inshinge "zisukuye" rwose kumasoko.icupa.Icyitegererezo cy'icyitegererezo no gushiramo uruhare mu isuzuma, ubwoko bwa J bwerekana icyitegererezo gikwiye guhitamo, ariko ntibikwiye kuri micro sample (hafi <500μL).Icyitegererezo B nacyo ni cyiza.Igishushanyo mbonera cya intubation y'imbere gifite intera nini ya porogaramu, ariko irazimvye.
Igihe cyo kohereza: Jun-17-2022