Amahame nuburyo bwo gusesengura kwinshi na Chromatografiya ya Liquid

Uburyo bwo gutandukanya amazi ya chromatografiya ashingiye ku itandukaniro riri hagati yimiterere yibigize muvanga ibyiciro byombi.

Ukurikije ibyiciro bitandukanye bihagaze, chromatografiya yamazi igabanyijemo ibice bikomeye byamazi ya chromatografiya, amazi ya chromatografiya yamazi na chromatografiya.Ikoreshwa cyane ni chromatografiya yamazi-ikomeye hamwe na silika gel nkuzuza hamwe na chromatografiya ihujwe na microsilica nka matrix.

Ukurikije uburyo bwicyiciro gihagaze, chromatografiya yamazi irashobora kugabanywa muburyo bwa chromatografiya, impapuro chromatografiya hamwe na chromatografi yoroheje.Ukurikije ubushobozi bwa adsorption, irashobora kugabanywa muri chromatografi ya adsorption, kugabana chromatografiya, ion guhana chromatografiya na chromatografi ya gel.

Mu myaka yashize, sisitemu yumuvuduko ukabije wamazi yongewe kuri sisitemu yamazi ya chromatografiya kugirango icyiciro cya mobile kigendagenda vuba munsi yumuvuduko mwinshi kugirango tunonosore ingaruka zo gutandukana, bityo imikorere-yo hejuru (izwi kandi nkumuvuduko ukabije) chromatografiya byagaragaye.

IGICE
01 Ihame ryisesengura ryinshi rya Chromatografiya

Kugereranya ukurikije ibintu byujuje ubuziranenge, bisukuye bisabwa nkibipimo;

Amazi ya chromatografiya yuzuye ni uburyo bwo kugereranya: ni ukuvuga, ingano ya analyte muruvange igereranijwe uhereye kumubare uzwi wicyitegererezo gisanzwe

IGICE
02 Shingiro rya Quantification by Liquid Chromatography

Ingano yikintu cyapimwe (W) iringaniza nigisubizo cyagaciro (A) (uburebure bwimpinga cyangwa agace ka mpinga), W = f × A.

Ikintu cyo gukosora ibintu (f): Nibisanzwe bihoraho muburyo bwo kubara, kandi ibisobanuro byumubiri ni umubare wibipimo byapimwe bigereranwa nagaciro kashubijwe (agace ka mpinga).

Ikintu cyo gukosora ibintu gishobora kuboneka uhereye ku mubare uzwi w'icyitegererezo gisanzwe n'agaciro kacyo.

Gupima agaciro k'ibisubizo bitazwi, kandi ingano yibigize irashobora kuboneka kubintu bikosora.

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03 Amagambo asanzwe mu isesengura ryinshi

Icyitegererezo (sample): igisubizo kirimo analyte yo gusesengura chromatografique.Igabanijwemo ingero zisanzwe kandi zitazwi.

Igipimo: Igicuruzwa cyera hamwe nibitekerezo bizwi.Icyitegererezo kitazwi (utabizi): Uruvange rugomba kugeragezwa.

Uburemere bw'icyitegererezo: Gupima umwimerere w'icyitegererezo ugomba gupimwa.

Dilution: Impamvu ya dilution ya sample itazwi.

Ibigize: impinga ya chromatografi igomba gusesengurwa mu bwinshi, ni ukuvuga analyte ibirimo bitazwi.

Umubare wibigize (umubare): ibirimo (cyangwa kwibanda) kubintu bigomba gupimwa.

Uburinganire: Uburyo bwo kubara bwo gupima agace ka mpinga ya chromatografique na mudasobwa.

Calibration curve: Umurongo ugororotse wibigize nibisubizo byagaciro, byashizweho uhereye kumubare uzwi wibintu bisanzwe, bikoreshwa mukumenya ibintu bitazwi bya analyte.

IGICE
04 Isesengura ryinshi rya Chromatografiya

1. Hitamo uburyo bwa chromatografique bukwiranye nisesengura ryinshi:

l Emeza impinga yibice byamenyekanye kandi ugere kumyanzuro (R) irenze 1.5

Menya ubudahangarwa (ubuziranenge) bwimpinga ya chromatografi yibice byageragejwe

Kugena imipaka yo kumenya no kugereranya uburyo;ibyiyumvo n'umurongo uringaniye

2. Shiraho kalibrasi yumurongo hamwe nicyitegererezo gisanzwe cyibitekerezo bitandukanye

3. Reba neza nuburyo bwuzuye muburyo bwo kubara

4. Koresha porogaramu ijyanye na chromatografiya kugirango ushyire mubikorwa icyegeranyo, gutunganya amakuru hamwe nibisubizo bya raporo

IGICE
05 Kumenyekanisha impinga zingana (qualitative)

Menya neza buri mpinga ya chromatografi igomba kugereranywa

Ubwa mbere, koresha icyitegererezo gisanzwe kugirango umenye igihe cyo kugumana (Rt) ya mpinga ya chromatografi kugirango igererwe.Mugereranije igihe cyo kugumana, shakisha ibice bihuye na buri mpinga ya chromatografique murugero rutazwi.Uburyo bwa chromatografique bufite ireme ni ukugereranya igihe cyo kugumana nicyitegererezo gisanzwe.Ibipimo ntibihagijeikindi cyemezo (qualitative)

1. Uburyo busanzwe bwo kongeramo

2. Koresha ubundi buryo icyarimwe: ubundi buryo bwa chromatografiya (hindura uburyo, nka: ukoresheje inkingi zitandukanye za chromatografiya), izindi disiketi (PDA: kugereranya spekiteri, gushakisha amasomero y'ibitabo; MS: isesengura rusange, isomero ryibitabo)

3. Ibindi bikoresho nuburyo

IGICE
06 Kwemeza impinga zingana

Emeza impinduramatwara ya chromatografique (isuku)

Menya neza ko hari ikintu kimwe cyapimwe munsi ya buri mpinga ya chromatografique

Reba kubangamira ibintu bifatanyiriza hamwe (umwanda)

Uburyo bwo Kwemeza Impinga ya Chromatografique (Isuku)

Kugereranya Spectrogrammes na Photodiode Matrix (PDA)

Kumenya neza

2996 Igitekerezo Cyiza

Uburyo butandukanye bukoreshwa mugice cya 07

Uburyo busanzwe bwo kugorora, bugabanijwe muburyo busanzwe bwo hanze nuburyo bwimbere:

1. Uburyo busanzwe bwo hanze: byinshi bikoreshwa mumazi ya chromatografiya

Urukurikirane rwibitegererezo rusanzwe rwibintu byateguwe byateguwe hifashishijwe ingero nziza yibintu bigomba gupimwa nkurugero rusanzwe.yatewe mu nkingi kugeza ku gisubizo cyayo (agace ka mpinga).
Mu ntera runaka, hari umurongo mwiza uhuza umurongo hagati yibitekerezo byurugero rusanzwe hamwe nigisubizo cyagaciro, aricyo W = f × A, kandi harakozwe umurongo usanzwe.

Mubihe bimwe byubushakashatsi, shyiramo icyitegererezo kitazwi kugirango ubone igisubizo cyibintu bigomba gupimwa.Ukurikije coefficient izwi f, ubunini bwibigize gupimwa burashobora kuboneka.

Ibyiza byuburyo busanzwe bwo hanze:imikorere yoroshye no kubara, nuburyo bukoreshwa muburyo bwo kubara;nta mpamvu ya buri kintu kigomba kumenyekana no gutorwa;icyitegererezo gisanzwe kirakenewe;ibipimo byo gupima icyitegererezo gisanzwe nicyitegererezo kitazwi bigomba kuba bihuye;ingano yo gutera inshinge igomba kuba itomoye.

Ibibi byuburyo busanzwe bwo hanze:Imiterere yubushakashatsi isabwa kuba hejuru, nkubwiyumvo bwa detector, igipimo cyurugendo, hamwe nibigize icyiciro kigendanwa ntibishobora guhinduka;ingano ya buri inshinge igomba kugira isubiramo ryiza.

2. Uburyo busanzwe bwimbere: byukuri, ariko bitera ibibazo, byinshi bikoreshwa muburyo busanzwe

Umubare uzwi wimiterere yimbere yongewe kumurongo kugirango ukore urwego ruvanze, kandi hateguwe urukurikirane rwibipimo byakazi byo kwibandaho.Ikigereranyo cyimyororokere yimiterere yimbere imbere murwego ruvanze ntigihinduka.Injira muri chromatografi yinkingi hanyuma ufate (icyitegererezo cyicyitegererezo cyumwanya / icyitegererezo cyimbere cyimbere) nkigisubizo cyagaciro.Ukurikije umurongo ugereranije hagati yigisubizo cyagaciro hamwe nubunini bwibikorwa bisanzwe, aribyo W = f × A, hakozwe umurongo usanzwe.

Umubare uzwi wimiterere yimbere yongewe kumurongo utazwi kandi winjijwe mumurongo kugirango ubone igisubizo cyibintu bigomba gupimwa.Ukurikije coefficient izwi f, ubunini bwibigize gupimwa burashobora kuboneka.

Ibiranga uburyo bwimbere bwimbere:Mugihe cyo gukora, icyitegererezo hamwe nibisanzwe byimbere bivangwa hamwe hanyuma bigaterwa mumurongo wa chromatografiya, mugihe rero igihe cyose igipimo cyumubare wibipimo byapimwe nibisanzwe byimbere mugisubizo kivanze gihoraho, ihinduka ryikitegererezo Ntabwo bizahindura ibisubizo byuzuye..Uburyo busanzwe bwimbere bukuraho ingaruka zubunini bwikitegererezo, ndetse nicyiciro kigendanwa na detector, kubwibyo birasobanutse neza kuruta uburyo busanzwe bwo hanze.

IGICE
08 Ibintu bigira ingaruka kubisesengura ryinshi

Ubusobanuro bubi bushobora guterwa na:

Guhuriza hamwe ahantu hahanamye, kubora byangiritse cyangwa umwanda watangijwe mugihe cyo gutegura icyitegererezo, vial sample idafunze, sample cyangwa solvent volatilisation, gutegura icyitegererezo kitari cyo, ibibazo byo gutera inshinge, gutegura neza imbere imbere

Impamvu zishoboka zo kudasobanuka neza:

Kwishyira hamwe kwimpinga zitari zo, gutera inshinge cyangwa gutera inshinge, kubora byintangarugero cyangwa umwanda watangijwe mugihe cyo gutegura icyitegererezo, ibibazo bya chromatografique, igisubizo cya detector cyangiritse